primary human dermal fibroblasts (hdf) cells Search Results


normal human dermal fibroblasts nhdf  (PromoCell)
98
PromoCell normal human dermal fibroblasts nhdf
Figure 2. A) Live/dead staining of normal human dermal fibroblasts <t>(NHDF)</t> with calcein-AM (green, live cells) and propidium iodide (red, dead cells) embedded in GelMA-free PEGMA/PEGDMA (Gel-N0) and GelMA-containing (Gel-N10) hydrogels on day 1, 7, 14 postencapsulation, followed by imaging with confocal microscopy (Leica TCS SPE, scale bar: 200 µm). B-i) NHDF embedded in Gel-N10 hydrogels on day 1 postencapsulation. Comparison of HepG2 cells encapsulated in (ii) Gel-N10 and (iii) Gel-N0 hydrogels (scale bar: 100 µm). C) Proliferation behavior of encapsulated HepG2 cells in Gel-N10 and Gel-N0 hydrogels over a period of 7 d assessed by PrestoBlue assay (n = 3). D) Toxicity evaluation of Gel-N10 photodegradation products by MTT proliferation assay with HeLa cells after 72 h exposure (n = 3). The concentration of nondiluted degradation products was 53 mg mL−1. Data are presented as mean ± SD and statistically evaluated by (C) Student’s t-test and (D) one-way ANOVA. *, **, *** represent p < 0.05, 0.01, and 0.001, respectively.
Normal Human Dermal Fibroblasts Nhdf, supplied by PromoCell, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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normal human dermal fibroblasts nhdf - by Bioz Stars, 2026-03
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human dermal fibroblasts  (Cell Applications Inc)
96
Cell Applications Inc human dermal fibroblasts
Figure 2. A) Live/dead staining of normal human dermal fibroblasts <t>(NHDF)</t> with calcein-AM (green, live cells) and propidium iodide (red, dead cells) embedded in GelMA-free PEGMA/PEGDMA (Gel-N0) and GelMA-containing (Gel-N10) hydrogels on day 1, 7, 14 postencapsulation, followed by imaging with confocal microscopy (Leica TCS SPE, scale bar: 200 µm). B-i) NHDF embedded in Gel-N10 hydrogels on day 1 postencapsulation. Comparison of HepG2 cells encapsulated in (ii) Gel-N10 and (iii) Gel-N0 hydrogels (scale bar: 100 µm). C) Proliferation behavior of encapsulated HepG2 cells in Gel-N10 and Gel-N0 hydrogels over a period of 7 d assessed by PrestoBlue assay (n = 3). D) Toxicity evaluation of Gel-N10 photodegradation products by MTT proliferation assay with HeLa cells after 72 h exposure (n = 3). The concentration of nondiluted degradation products was 53 mg mL−1. Data are presented as mean ± SD and statistically evaluated by (C) Student’s t-test and (D) one-way ANOVA. *, **, *** represent p < 0.05, 0.01, and 0.001, respectively.
Human Dermal Fibroblasts, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pooled human dermal fibroblasts hdfp  (CELLnTEC Advanced Cell Systems AG)
90
CELLnTEC Advanced Cell Systems AG pooled human dermal fibroblasts hdfp
Figure 2. A) Live/dead staining of normal human dermal fibroblasts <t>(NHDF)</t> with calcein-AM (green, live cells) and propidium iodide (red, dead cells) embedded in GelMA-free PEGMA/PEGDMA (Gel-N0) and GelMA-containing (Gel-N10) hydrogels on day 1, 7, 14 postencapsulation, followed by imaging with confocal microscopy (Leica TCS SPE, scale bar: 200 µm). B-i) NHDF embedded in Gel-N10 hydrogels on day 1 postencapsulation. Comparison of HepG2 cells encapsulated in (ii) Gel-N10 and (iii) Gel-N0 hydrogels (scale bar: 100 µm). C) Proliferation behavior of encapsulated HepG2 cells in Gel-N10 and Gel-N0 hydrogels over a period of 7 d assessed by PrestoBlue assay (n = 3). D) Toxicity evaluation of Gel-N10 photodegradation products by MTT proliferation assay with HeLa cells after 72 h exposure (n = 3). The concentration of nondiluted degradation products was 53 mg mL−1. Data are presented as mean ± SD and statistically evaluated by (C) Student’s t-test and (D) one-way ANOVA. *, **, *** represent p < 0.05, 0.01, and 0.001, respectively.
Pooled Human Dermal Fibroblasts Hdfp, supplied by CELLnTEC Advanced Cell Systems AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human dermal fibroblast (hdf) cells  (HiMedia Laboratories)
90
HiMedia Laboratories human dermal fibroblast (hdf) cells
Figure 2. A) Live/dead staining of normal human dermal fibroblasts <t>(NHDF)</t> with calcein-AM (green, live cells) and propidium iodide (red, dead cells) embedded in GelMA-free PEGMA/PEGDMA (Gel-N0) and GelMA-containing (Gel-N10) hydrogels on day 1, 7, 14 postencapsulation, followed by imaging with confocal microscopy (Leica TCS SPE, scale bar: 200 µm). B-i) NHDF embedded in Gel-N10 hydrogels on day 1 postencapsulation. Comparison of HepG2 cells encapsulated in (ii) Gel-N10 and (iii) Gel-N0 hydrogels (scale bar: 100 µm). C) Proliferation behavior of encapsulated HepG2 cells in Gel-N10 and Gel-N0 hydrogels over a period of 7 d assessed by PrestoBlue assay (n = 3). D) Toxicity evaluation of Gel-N10 photodegradation products by MTT proliferation assay with HeLa cells after 72 h exposure (n = 3). The concentration of nondiluted degradation products was 53 mg mL−1. Data are presented as mean ± SD and statistically evaluated by (C) Student’s t-test and (D) one-way ANOVA. *, **, *** represent p < 0.05, 0.01, and 0.001, respectively.
Human Dermal Fibroblast (Hdf) Cells, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lyophilized porcine dermal skin alloask d  (Taiho Pharmaceutical)
90
Taiho Pharmaceutical lyophilized porcine dermal skin alloask d
Figure 2. A) Live/dead staining of normal human dermal fibroblasts <t>(NHDF)</t> with calcein-AM (green, live cells) and propidium iodide (red, dead cells) embedded in GelMA-free PEGMA/PEGDMA (Gel-N0) and GelMA-containing (Gel-N10) hydrogels on day 1, 7, 14 postencapsulation, followed by imaging with confocal microscopy (Leica TCS SPE, scale bar: 200 µm). B-i) NHDF embedded in Gel-N10 hydrogels on day 1 postencapsulation. Comparison of HepG2 cells encapsulated in (ii) Gel-N10 and (iii) Gel-N0 hydrogels (scale bar: 100 µm). C) Proliferation behavior of encapsulated HepG2 cells in Gel-N10 and Gel-N0 hydrogels over a period of 7 d assessed by PrestoBlue assay (n = 3). D) Toxicity evaluation of Gel-N10 photodegradation products by MTT proliferation assay with HeLa cells after 72 h exposure (n = 3). The concentration of nondiluted degradation products was 53 mg mL−1. Data are presented as mean ± SD and statistically evaluated by (C) Student’s t-test and (D) one-way ANOVA. *, **, *** represent p < 0.05, 0.01, and 0.001, respectively.
Lyophilized Porcine Dermal Skin Alloask D, supplied by Taiho Pharmaceutical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human dermal fibroblasts  (Procell Inc)
90
Procell Inc human dermal fibroblasts
Figure 2. A) Live/dead staining of normal human dermal fibroblasts <t>(NHDF)</t> with calcein-AM (green, live cells) and propidium iodide (red, dead cells) embedded in GelMA-free PEGMA/PEGDMA (Gel-N0) and GelMA-containing (Gel-N10) hydrogels on day 1, 7, 14 postencapsulation, followed by imaging with confocal microscopy (Leica TCS SPE, scale bar: 200 µm). B-i) NHDF embedded in Gel-N10 hydrogels on day 1 postencapsulation. Comparison of HepG2 cells encapsulated in (ii) Gel-N10 and (iii) Gel-N0 hydrogels (scale bar: 100 µm). C) Proliferation behavior of encapsulated HepG2 cells in Gel-N10 and Gel-N0 hydrogels over a period of 7 d assessed by PrestoBlue assay (n = 3). D) Toxicity evaluation of Gel-N10 photodegradation products by MTT proliferation assay with HeLa cells after 72 h exposure (n = 3). The concentration of nondiluted degradation products was 53 mg mL−1. Data are presented as mean ± SD and statistically evaluated by (C) Student’s t-test and (D) one-way ANOVA. *, **, *** represent p < 0.05, 0.01, and 0.001, respectively.
Human Dermal Fibroblasts, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human dermal fibroblasts - by Bioz Stars, 2026-03
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human primary dermal fibroblast cell line gmo1651  (Coriell Institute for Medical Research)
90
Coriell Institute for Medical Research human primary dermal fibroblast cell line gmo1651
Isolation of SKPs from control and HGPS fibroblasts. ( a ) Panel showing the protocol for SKP isolation. Briefly, fibroblasts were pelleted and treated with HBSS buffer (pH 5.7) for 30 min at 37 °C. Cells were cultured in SKP media containing DMEM low glucose, EGF, FGF, and B27. The flasks were agitated daily, and the spheroids were harvested at day 4 for analysis. ( b ) SKP formation from both control (GMO1651c, <t>GMO5565)</t> and HGPS (HGADFN127, HGADFN003) fibroblasts with 5 and 30% senescence (SNS). ( c , d ) Quantification of the number and the diameter of the spheroids from control and HGPS fibroblast cultures with 5 and 30% SNS at day 4. Values are presented as mean ± SD ( n = 3), not significant (ns), * p < 0.05, ( c , d ) unpaired t -test. HBSS: Hank’s Balanced Salt Solution, DMEM: Dulbecco′s modified Eagle medium, EGF: epidermal growth factor, FGF: fibroblast growth factor, SKPs: skin-derived precursor cells, SNS: senescence.
Human Primary Dermal Fibroblast Cell Line Gmo1651, supplied by Coriell Institute for Medical Research, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human primary dermal fibroblast cell line gmo1651 - by Bioz Stars, 2026-03
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hdf (human dermal fibroblast) cell line  (National Centre for Cell Science)
90
National Centre for Cell Science hdf (human dermal fibroblast) cell line
Isolation of SKPs from control and HGPS fibroblasts. ( a ) Panel showing the protocol for SKP isolation. Briefly, fibroblasts were pelleted and treated with HBSS buffer (pH 5.7) for 30 min at 37 °C. Cells were cultured in SKP media containing DMEM low glucose, EGF, FGF, and B27. The flasks were agitated daily, and the spheroids were harvested at day 4 for analysis. ( b ) SKP formation from both control (GMO1651c, <t>GMO5565)</t> and HGPS (HGADFN127, HGADFN003) fibroblasts with 5 and 30% senescence (SNS). ( c , d ) Quantification of the number and the diameter of the spheroids from control and HGPS fibroblast cultures with 5 and 30% SNS at day 4. Values are presented as mean ± SD ( n = 3), not significant (ns), * p < 0.05, ( c , d ) unpaired t -test. HBSS: Hank’s Balanced Salt Solution, DMEM: Dulbecco′s modified Eagle medium, EGF: epidermal growth factor, FGF: fibroblast growth factor, SKPs: skin-derived precursor cells, SNS: senescence.
Hdf (Human Dermal Fibroblast) Cell Line, supplied by National Centre for Cell Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hdf (human dermal fibroblast) cell line - by Bioz Stars, 2026-03
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human dermal fibroblast (hdf) cell line #ag21708  (Coriell Institute for Medical Research)
90
Coriell Institute for Medical Research human dermal fibroblast (hdf) cell line #ag21708
Isolation of SKPs from control and HGPS fibroblasts. ( a ) Panel showing the protocol for SKP isolation. Briefly, fibroblasts were pelleted and treated with HBSS buffer (pH 5.7) for 30 min at 37 °C. Cells were cultured in SKP media containing DMEM low glucose, EGF, FGF, and B27. The flasks were agitated daily, and the spheroids were harvested at day 4 for analysis. ( b ) SKP formation from both control (GMO1651c, <t>GMO5565)</t> and HGPS (HGADFN127, HGADFN003) fibroblasts with 5 and 30% senescence (SNS). ( c , d ) Quantification of the number and the diameter of the spheroids from control and HGPS fibroblast cultures with 5 and 30% SNS at day 4. Values are presented as mean ± SD ( n = 3), not significant (ns), * p < 0.05, ( c , d ) unpaired t -test. HBSS: Hank’s Balanced Salt Solution, DMEM: Dulbecco′s modified Eagle medium, EGF: epidermal growth factor, FGF: fibroblast growth factor, SKPs: skin-derived precursor cells, SNS: senescence.
Human Dermal Fibroblast (Hdf) Cell Line #Ag21708, supplied by Coriell Institute for Medical Research, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human dermal fibroblast (hdf) cell line #ag21708 - by Bioz Stars, 2026-03
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primary human dermal fibroblast cells  (GlobalStem)
90
GlobalStem primary human dermal fibroblast cells
Isolation of SKPs from control and HGPS fibroblasts. ( a ) Panel showing the protocol for SKP isolation. Briefly, fibroblasts were pelleted and treated with HBSS buffer (pH 5.7) for 30 min at 37 °C. Cells were cultured in SKP media containing DMEM low glucose, EGF, FGF, and B27. The flasks were agitated daily, and the spheroids were harvested at day 4 for analysis. ( b ) SKP formation from both control (GMO1651c, <t>GMO5565)</t> and HGPS (HGADFN127, HGADFN003) fibroblasts with 5 and 30% senescence (SNS). ( c , d ) Quantification of the number and the diameter of the spheroids from control and HGPS fibroblast cultures with 5 and 30% SNS at day 4. Values are presented as mean ± SD ( n = 3), not significant (ns), * p < 0.05, ( c , d ) unpaired t -test. HBSS: Hank’s Balanced Salt Solution, DMEM: Dulbecco′s modified Eagle medium, EGF: epidermal growth factor, FGF: fibroblast growth factor, SKPs: skin-derived precursor cells, SNS: senescence.
Primary Human Dermal Fibroblast Cells, supplied by GlobalStem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary human dermal fibroblast cells - by Bioz Stars, 2026-03
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human dermal fibroblasts (hdf) cells  (Greiner Bio)
90
Greiner Bio human dermal fibroblasts (hdf) cells
Isolation of SKPs from control and HGPS fibroblasts. ( a ) Panel showing the protocol for SKP isolation. Briefly, fibroblasts were pelleted and treated with HBSS buffer (pH 5.7) for 30 min at 37 °C. Cells were cultured in SKP media containing DMEM low glucose, EGF, FGF, and B27. The flasks were agitated daily, and the spheroids were harvested at day 4 for analysis. ( b ) SKP formation from both control (GMO1651c, <t>GMO5565)</t> and HGPS (HGADFN127, HGADFN003) fibroblasts with 5 and 30% senescence (SNS). ( c , d ) Quantification of the number and the diameter of the spheroids from control and HGPS fibroblast cultures with 5 and 30% SNS at day 4. Values are presented as mean ± SD ( n = 3), not significant (ns), * p < 0.05, ( c , d ) unpaired t -test. HBSS: Hank’s Balanced Salt Solution, DMEM: Dulbecco′s modified Eagle medium, EGF: epidermal growth factor, FGF: fibroblast growth factor, SKPs: skin-derived precursor cells, SNS: senescence.
Human Dermal Fibroblasts (Hdf) Cells, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human dermal fibroblasts (hdf) cells - by Bioz Stars, 2026-03
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human dermal fibroblast (hdf) cells  (Vala Sciences)
90
Vala Sciences human dermal fibroblast (hdf) cells
Isolation of SKPs from control and HGPS fibroblasts. ( a ) Panel showing the protocol for SKP isolation. Briefly, fibroblasts were pelleted and treated with HBSS buffer (pH 5.7) for 30 min at 37 °C. Cells were cultured in SKP media containing DMEM low glucose, EGF, FGF, and B27. The flasks were agitated daily, and the spheroids were harvested at day 4 for analysis. ( b ) SKP formation from both control (GMO1651c, <t>GMO5565)</t> and HGPS (HGADFN127, HGADFN003) fibroblasts with 5 and 30% senescence (SNS). ( c , d ) Quantification of the number and the diameter of the spheroids from control and HGPS fibroblast cultures with 5 and 30% SNS at day 4. Values are presented as mean ± SD ( n = 3), not significant (ns), * p < 0.05, ( c , d ) unpaired t -test. HBSS: Hank’s Balanced Salt Solution, DMEM: Dulbecco′s modified Eagle medium, EGF: epidermal growth factor, FGF: fibroblast growth factor, SKPs: skin-derived precursor cells, SNS: senescence.
Human Dermal Fibroblast (Hdf) Cells, supplied by Vala Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human dermal fibroblast (hdf) cells - by Bioz Stars, 2026-03
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Image Search Results


Figure 2. A) Live/dead staining of normal human dermal fibroblasts (NHDF) with calcein-AM (green, live cells) and propidium iodide (red, dead cells) embedded in GelMA-free PEGMA/PEGDMA (Gel-N0) and GelMA-containing (Gel-N10) hydrogels on day 1, 7, 14 postencapsulation, followed by imaging with confocal microscopy (Leica TCS SPE, scale bar: 200 µm). B-i) NHDF embedded in Gel-N10 hydrogels on day 1 postencapsulation. Comparison of HepG2 cells encapsulated in (ii) Gel-N10 and (iii) Gel-N0 hydrogels (scale bar: 100 µm). C) Proliferation behavior of encapsulated HepG2 cells in Gel-N10 and Gel-N0 hydrogels over a period of 7 d assessed by PrestoBlue assay (n = 3). D) Toxicity evaluation of Gel-N10 photodegradation products by MTT proliferation assay with HeLa cells after 72 h exposure (n = 3). The concentration of nondiluted degradation products was 53 mg mL−1. Data are presented as mean ± SD and statistically evaluated by (C) Student’s t-test and (D) one-way ANOVA. *, **, *** represent p < 0.05, 0.01, and 0.001, respectively.

Journal: Advanced healthcare materials

Article Title: Designing Inherently Photodegradable Cell-Adhesive Hydrogels for 3D Cell Culture.

doi: 10.1002/adhm.202100632

Figure Lengend Snippet: Figure 2. A) Live/dead staining of normal human dermal fibroblasts (NHDF) with calcein-AM (green, live cells) and propidium iodide (red, dead cells) embedded in GelMA-free PEGMA/PEGDMA (Gel-N0) and GelMA-containing (Gel-N10) hydrogels on day 1, 7, 14 postencapsulation, followed by imaging with confocal microscopy (Leica TCS SPE, scale bar: 200 µm). B-i) NHDF embedded in Gel-N10 hydrogels on day 1 postencapsulation. Comparison of HepG2 cells encapsulated in (ii) Gel-N10 and (iii) Gel-N0 hydrogels (scale bar: 100 µm). C) Proliferation behavior of encapsulated HepG2 cells in Gel-N10 and Gel-N0 hydrogels over a period of 7 d assessed by PrestoBlue assay (n = 3). D) Toxicity evaluation of Gel-N10 photodegradation products by MTT proliferation assay with HeLa cells after 72 h exposure (n = 3). The concentration of nondiluted degradation products was 53 mg mL−1. Data are presented as mean ± SD and statistically evaluated by (C) Student’s t-test and (D) one-way ANOVA. *, **, *** represent p < 0.05, 0.01, and 0.001, respectively.

Article Snippet: Normal human dermal fibroblasts (NHDF), human liver cancer cells (HepG2), and HeLa cells were purchased from PromoCell GmbH (Heidelberg, Germany).

Techniques: Staining, Imaging, Confocal Microscopy, Comparison, Prestoblue Assay, Proliferation Assay, Concentration Assay

Isolation of SKPs from control and HGPS fibroblasts. ( a ) Panel showing the protocol for SKP isolation. Briefly, fibroblasts were pelleted and treated with HBSS buffer (pH 5.7) for 30 min at 37 °C. Cells were cultured in SKP media containing DMEM low glucose, EGF, FGF, and B27. The flasks were agitated daily, and the spheroids were harvested at day 4 for analysis. ( b ) SKP formation from both control (GMO1651c, GMO5565) and HGPS (HGADFN127, HGADFN003) fibroblasts with 5 and 30% senescence (SNS). ( c , d ) Quantification of the number and the diameter of the spheroids from control and HGPS fibroblast cultures with 5 and 30% SNS at day 4. Values are presented as mean ± SD ( n = 3), not significant (ns), * p < 0.05, ( c , d ) unpaired t -test. HBSS: Hank’s Balanced Salt Solution, DMEM: Dulbecco′s modified Eagle medium, EGF: epidermal growth factor, FGF: fibroblast growth factor, SKPs: skin-derived precursor cells, SNS: senescence.

Journal: Cells

Article Title: Impact of Progerin Expression on Adipogenesis in Hutchinson—Gilford Progeria Skin-Derived Precursor Cells

doi: 10.3390/cells10071598

Figure Lengend Snippet: Isolation of SKPs from control and HGPS fibroblasts. ( a ) Panel showing the protocol for SKP isolation. Briefly, fibroblasts were pelleted and treated with HBSS buffer (pH 5.7) for 30 min at 37 °C. Cells were cultured in SKP media containing DMEM low glucose, EGF, FGF, and B27. The flasks were agitated daily, and the spheroids were harvested at day 4 for analysis. ( b ) SKP formation from both control (GMO1651c, GMO5565) and HGPS (HGADFN127, HGADFN003) fibroblasts with 5 and 30% senescence (SNS). ( c , d ) Quantification of the number and the diameter of the spheroids from control and HGPS fibroblast cultures with 5 and 30% SNS at day 4. Values are presented as mean ± SD ( n = 3), not significant (ns), * p < 0.05, ( c , d ) unpaired t -test. HBSS: Hank’s Balanced Salt Solution, DMEM: Dulbecco′s modified Eagle medium, EGF: epidermal growth factor, FGF: fibroblast growth factor, SKPs: skin-derived precursor cells, SNS: senescence.

Article Snippet: The human primary dermal fibroblast cell lines, GMO5565 (3-year-old male), GMO1651 (13-year-old female) and GMO5567A (12-year-old male) were all obtained from the Coriell Institute for Medical Research (Camden, NJ, USA).

Techniques: Isolation, Control, Cell Culture, Modification, Derivative Assay